Part:BBa_M36580:Experience
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Stanford Location
Glycerol Stocks
Barcode #: 0133010047
Plasmid Name: qsc102
Antibiotic resistance: kanamycin
DNA 2.0 gene #: 232006
Organism expressed in: E. coli
Sensor
Barcode #: 0133010047
Plasmid Names (double transformed): LasR + qsc102
Antibiotic resistance: ampicillin + kanamycin
DNA 2.0 gene #: 232005; 232006
Organism expressed in: E. coli
Double Plasmid Actuator/Sensor
Applications of BBa_M36580
Used in conjunction with BBa_M36575 in BIOE44 at Stanford in attempts to create a P. aeruginosa biosensor.
User Reviews
UNIQ294b2fd61036b57d-partinfo-00000000-QINU UNIQ294b2fd61036b57d-partinfo-00000001-QINU
We have little data on the functionality of this construct since it was used in conjunction with BBa_M36575, which proved inviable. However, what we do know is that a low amount of fluorescence was observed in all cells with the qsc102 plasmid, despite a lack of LasR presence (Graph 1). The promoter is likely leaky, and transcribed the GFP gene following it even without presence of the LasR-PAI1 complex.
Graph 1. Normalized fluorescence levels for GFP expression in the double-transformed E. coli